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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
26/09/2014 |
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Actualizado : |
18/03/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
IBÁÑEZ, F.; REZANO, A.; FREDES, A.; RODRIGUEZ, G.; REGGIO, A.; VILARÓ, F. |
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Afiliación : |
FACUNDO IBÁÑEZ SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANABELA REZANO MATKOVICH, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ALEJANDRO DAVID FREDES SIVOPLAS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; GUSTAVO ADOLFO RODRIGUEZ TEXEIRA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ADRIANA MARIA REGGIO BIGANZOLI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FRANCISCO LUIS VILARO PAREJA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Development of a rapid microplate assay for determining pungency in onion breeding program. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Acta Horticulturae (ISHS), 2012, no. 935, p. 61-65. |
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ISSN : |
0567-7572 |
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DOI : |
10.17660/ActaHortic.2012.935.8 |
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Idioma : |
Inglés |
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Notas : |
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding. |
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Contenido : |
ABSTRACT.
Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95). |
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Thesagro : |
AJO. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02138naa a2200229 a 4500
001 1050611
005 2022-03-18
008 2012 bl uuuu u00u1 u #d
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2012.935.8$2DOI
100 1 $aIBÁÑEZ, F.
245 $aDevelopment of a rapid microplate assay for determining pungency in onion breeding program.$h[electronic resource]
260 $c2012
500 $aXXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding.
520 $aABSTRACT. Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95).
650 $aAJO
700 1 $aREZANO, A.
700 1 $aFREDES, A.
700 1 $aRODRIGUEZ, G.
700 1 $aREGGIO, A.
700 1 $aVILARÓ, F.
773 $tActa Horticulturae (ISHS), 2012, no. 935, p. 61-65.
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| Registros recuperados : 4 | |
| 1. |  | BODEN, S.A.; MCINTOSH, R.A.; UAUY, C.; KRATTINGER, S.G.; DUBCOVSKY, J.; ROGERS, W.J.; XIA, X.C.; BADAEVA, E.D.; BENTLEY, A.R.; BROWN-GUEDIRA, G.; CACCAMO, M.; CATTIVELLI, L.; CHHUNEJA, P.; COCKRAM, J.; CONTRERAS-MOREIRA, B.; DREISIGACKER, S.; EDWARDS, D.; GONZÁLEZ, F.G.; GUZMÁN, C.; IKEDA, T.M.; KARSAI, I.; NASUDA, S.; POZNIAK, C.; PRINS, R.; SEN, T.Z.; SILVA, P.; SIMKOVA, H.; ZHANG, Y. Updated guidelines for gene nomenclature in wheat. Theoretical and Applied Genetics, 2023, Volume 136, Issue 4, article 72. OPEN ACCESS. Doi: https://doi.org/10.1007/s00122-023-04253-w Article history: Received 25 May 2022, Accepted 10 October 2022, Published 23 March 2023. -- Communicated by Peter Langridge. -- Correspondence authors: Boden, S.A.; School of Agriculture, Food and Wine, Waite Research Institute,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 2. | | LANGRIDGE, P.; ALAUX, M.; ALMEIDA, N.F.; AMMAR, K.; BAUM, M.; BEKKAOUI, F.; BENTLEY, A.R.; BERES, B.L.; BERGER, B.; BRAUN, H.-J.; BROWN-GUEDIRA, G.; BURT, C.J.; CACCAMO, M.J.; CATTIVELLI, L.; CHARMET, G.; CIVÁN, P.; CLOUTIER, S.; COHAN, J-P.; DEVAUX, P.; DOOHAN, F.M.; DRECCER, M.F.; FERRAHI, M.; GERMAN, S.; GOODWIN, S.B.; GRIFFITHS, S.; GUZMÁN, C.; HANDA, H.; HAWKESFORD, M.J.; HE, Z.; HUTTNER, E.; IKEDA, T.M.; KILIAN, B.; KING, I.P.; KING, J.; KIRKEGAARD, J.A.; LAGE, J.; LE GOUIS, J.; MONDAL, S.; MULLINS, E.; ORDON, F.; ORTIZ-MONASTERIO, J.I.; ÖZKAN, H.; ÖZTÜRK, I.; PEREYRA, S.; POZNIAK, C.J.; QUESNEVILLE, H.; QUINCKE, M.; REBETZKE, G.J.; CHRISTOPH REIF, J.; SAAVEDRA-BRAVO, T.; SCHURR, U.; SHARMA, S.; SINGH, S.K.; SINGH, R.P.; SNAPE, J.W.; TADESSE, W.; TSUJIMOTO, H.; TUBEROSA, R.; WILLIS, T.G.; ZHANG, X. Meeting the challenges facing wheat production: the strategic research agenda of the Global Wheat Initiative. Agronomy, 2022, volume 12, issue 11, 2767. OPEN ACCESS. doi: https://doi.org/10.3390/agronomy12112767 Article history: Received 26 September 2022; Revised 28 October 2022; Accepted 29 October 2022; Published 7 November 2022. -- Academic Editor: Andreas Katsiotis. -- Corresponding author: Langridge, P.; School of Agriculture, Food and...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 3. | | LADO, B.; BATTENFIELD, S.; SILVA, P.; QUINCKE, M.; GUZMAN, C.; SINGH, R.P.; DREISIGACKER, S.; PEÑA, J.; FRITZ, A.; POLAND, J.; GUTIERREZ, L. Comparing strategies to select crosses using genomic prediction in two wheat breeding programs. In: International Wheat Genetics Symposium, 12, Tulln, Austria; April 23-28, 2017; BOKU: University of Natural Resources and Life Sciences, Vienna, Austria. p.88-90.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 4. | | LADO, B.; BATTENFIELD, S. D.; GUZMÁN, C.; QUINCKE, M.; SINGH, R. P.; DREISIGACKER, S.; PEÑA, R. J.; FRITZ, AL.; SILVA, P.; POLAND, J.; GUTIÉRREZ, L. Strategies for selecting crosses using genomic prediction in two wheat breeding programs. The Plant Genome, 2017, v.10, Issue 2, 12p. OPEN ACCESS Article history: Received: Dec 14, 2016 // Accepted: Mar 18, 2017 // Published: July 6, 2017.
B. Lado and S. Battenfield contributed equally.Assigned to Associate Editor Nicholas Tinker.
This is an open access article distributed under...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 4 | |
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